4E-BP1/2/3 Phospho (pT45) (EIF4EBP1)

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4E-BP1/2/3 Phospho (pT45) (EIF4EBP1)

Rabbit Monoclonal Antibody

别名：	EIF4EBP1 antibody, 4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, MGC4316 antibody, PHAS-I antibody, Eukaryotic translation initiation factor 4E-binding protein 1 antibody, Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
Cas号：	--	M D L：	--
分子式：	--	分子量：	--

货号	品名	规格	包装	单价	货期	库存
JD1227177497	4E-BP1/2/3 Phospho (pT45) (EIF4EBP1)	--	100ul	0元	现货	3天

物流提示：偏远地区如新疆西藏宁夏甘肃等地可能无法运送液体，下单前请联系客服。

性状：

【反应特异性】Human , Mouse
【克隆 ID】EPR2169Y
【用途】WB , IHC , ICC , IP
【抗原分子量】15 - 20kDa

质量标准：

【产品介绍】4E-BP1 (eIF4E-binding protein) also known
as PHAS, is a 10-12 kDa acidic protein that compete
with eIF4G for binding of eiF4E to the mRNA 5 cap
structure (1). Binding of the 4E-BPs to eIF4E is
reversible and is dependent on the phosphorylation
status of 4E-BP. Non-phosphorylated 4E-BP1 will
bind strongly to eiF4E while, the phosphorylated form
will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2
are known kinases capable of inactivating 4E-BP1 binding
to eIF4E by phosphorylating either threonines 35, 45, 69
or serine 64. Although, not all phosphorylation
events equally block the 4EBP1-eIF4E interaction (3-4)

Recommended Applications

WB, IHC, ICC, IP
Applications and Recommended Dilution Factors

WB: 1:5000
IHC: 1:50
ICC: 1:100
IP: 1:10
Species Reactivity *

Human, Mouse

*Cross reactivity determined by western blot only.

Specificity

A phospho specific peptide corresponding to
residues surrounding threonine 45 was used as an
immunogen. This antibody detects 4E-BP1/2/3
phosphorylated at threonine 45.

贮存：

Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.