AMPK alpha 1 Phospho (pT183) / AMPK alpha 2 Phospho (pT172) (PRKAA1)

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AMPK alpha 1 Phospho (pT183) / AMPK alpha 2 Phospho (pT172) (PRKAA1)

Rabbit Monoclonal Antibody

别名：	PRKAA1 antibody, AMPK antibody, AMPKa1 antibody, MGC33776 antibody, MGC57364 antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, antibody
Cas号：	--	M D L：	--
分子式：	--	分子量：	--

货号	品名	规格	包装	单价	货期	库存
JD1227177530	AMPK alpha 1 Phospho (pT183)	--	100ul	0元	现货	3天

物流提示：偏远地区如新疆西藏宁夏甘肃等地可能无法运送液体，下单前请联系客服。

性状：

【反应特异性】Human , Mouse , Rat
【克隆 ID】EPR5683
【用途】WB , IP
【抗原分子量】62/63kDa

质量标准：

【产品介绍】The 5-AMP-activated protein kinase (AMPK),
a member of the SNF1 (sucrose nonfermentor) kinase
family (1) AMPK is a heterotrimeric protein comprising
α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The
alpha subunit is the catalytic subunit (which
requires phosphorylation to be active) (2-3). Beta and
gamma are noncatalytic subunits, although they have
been found to interact with the active subunit in
liver. AMPK regulates fatty acid and sterol synthesis
by phosphorylation of acetyl-CoA as well as
cholesterol synthesis via phosphorylation and
inactivation of hydroxymethylglutaryl-CoA reductase
(4). AMPK also appears to play a role in protecting
cells from stresses that cause ATP depletion by
switching off ATP-consuming biosynthetic pathways.

Recommended Applications

WB, IP
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IP: 1:10 - 100
Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

Products Data
Western blot analysis on 293 cell lysates
using anti-Phospho-AMPK alpha 1/AMPK alpha 2
(pT183/pT172) RabMAb (cat. #3930-1). Cells were
either (A) untreated or (B) treated with Lambda phosphatase.

Specificity

A phospho specific peptide corresponding to
residues surrounding Threonine 183 of human AMPK alpha 1
and Threonine 172 of human AMPK alpha 2 was used as
an immunogen. This antibody only detects AMPK alpha
1 phosphorylated at Threonine 183 and AMPK alpha
2 phosphorylated Threonine 172.

贮存：

Storage Condition and Buffer

Store at -20 °C. Antibody buffer, 0.01% sodium azide,
50% Glycerol and 0.05% BSA. Stable for 12 months from date
of receipt