Tubulin beta-III_珈得尔化学试剂

【产品介绍】Microtubules are essential parts in
eukaryotic cell structures, transp o r tation, a n d
mitosis a n d consists mainly of 2 soluble protein
subunits, alpha a n d beta tubulin. Beta-tubulin binds
to alpha tubulin to f o r m tubulin heterodimer which
is post-translationally modified (1). The tubulin
dimer complex binds to GTP a n d assembles onto the
positive ends of microtubules. After inc o r p o r
ation into the microtubules, bound GTP is hydrolyzed by
beta tubulin. The stability of the dimer in the
microtubules is depended on presence of beta tubulin,
where dimer with GTP bound beta-tubulin is stable
to microtubule inc o r p o r ation (2). Mutation
on beta-tubulin has been linked to anti-cancer
drug resistance in lung cancers (3).

Recommended Applications

WB, IHC, ICC, FC, IP
Applications and Recommended Dilution Factors

WB: 1:20,000
IHC: 1:250 - 500
ICC: 1:100 - 250
FC: 1:10
IP: 1:50
Species Reactivity *

Human, Mouse, Rat

* *Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on HeLa cell lysate using
anti- Beta-Tubulin RabMAb (cat. # 1879-1), dilution 1:20,000.

B. Immunohistochemical analysis of paraffin-embedded
human gastric carcinoma using anti-Beta-Tubulin RabMAb
(cat# 1879-1)

C. Imunofluorescent staining of HeLa cells using
anti- Beta-Tubulin RabMAb (cat# 1879-1)

Specificity

A synthetic peptide corresponding to residues near
the C-term of human β-Tubulin was used as immunogen.

贮存：

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol,
0.01% sodium azide and 0.05% BSA.