Chromogranin A (CHGA)_珈得尔化学试剂

【产品介绍】Chromogranin A (CgA) is an 86 kDa protein
that is the major member of the granin family of
acidic secretory glycoproteins located in
neuroendocrine cells. CgA is believed to play a role
in targeting peptide hormones and neurotransmitters
to granules of the regulated pathways and inhibit
hormone and neurotransmitter release (1). Also,
the widespread distribution of CgA has made the
measurement of circulating immunoreactive CgA a
valuable tool in the diagnosis of neuroendocrine
neoplasia, and CgA immunohistochemistry can help to
identify the neuroendocrine nature of tumors. The
N-terminal domain of CgA inhibits tumor necrosis factor
α (TNFα) induced gap formation in human umbilical
venous endothelial cells (2). CgA levels which
reflect neuroendocrine differentiation of
prostatic carcinoma may have a diagnostic, therapeutic
and prognostic role in the management of prostate
cancer patients (3).

Recommended Applications

WB, IHC, IP
Applications and Recommended Dilution Factors

WB: 1:1,000,000
IHC: 1:250-500
IP: 1:30
Species Reactivity *

Human

*Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on PC12 cell lysate of
using anti-Chromogranin A Rabbit Monoclonal (cat. # 1809-1), dilution 1:1,000,000

B. Immunohistochemical analysis of paraffin-embedded
human pancreatic tissue using anti-Chromogranin A
Rabbit Monoclonal (cat. #1809-1).

Specificity

A synthetic peptide corresponding to residues near
the C-terminus of human Chromogranin A was used as
an immunogen.

贮存：

Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.