Cyclin E1 Phospho (pT77) (CCNE1)_珈得尔化学试剂

【产品介绍】Cyclin E1 is a member of the cyclin E
family that can associate with and activate
cyclin-dependent kinase Cdk2. Expression of cyclin E1
is essential for the control of the cell cycle at the
late G1 and early S phase (1). The cyclin E/Cdk2
complex plays an essential and limiting role in
the transition between G1 and S phase as well as
the initiation of DNA replication (2) Ubiquination by
the Cul-3 pathway and by Fbw7 regulates cyclin E1 levels
and is critically important in normal cells. Abnormally
high levels of cyclin E expression have frequently
been observed in human cancers (3). Moreover, high cyclin
E1 levels correlate with increased tumor aggression and
poor prognosis (4).

Recommended Applications

WB, IHC, ICC
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:250 - 500
Species Reactivity *

Human

*Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on JAR cell lysates using
anti-Phospho-Cyclin E1 (pT77) RabMAb (cat. #5105-1).
Cells were either (A) untreated or (B) treated with
Lambda Phosphatase.

Immunohistochemical analysis of paraffin-embedded
human colon tissue using anti-Phospho-Cyclin E1
(pT77) RabMAb (cat. #5105-1).

Specificity

A phospho specific peptide corresponding to
residues surrounding Threonine 77 of human Cyclin E1
was used as an immunogen. This antibody only detects
Cyclin E1 phosphorylated at Threonine 77.

贮存：

Storage Condition and Buffer

Store at -20 °C. Antibody buffer, 0.01% sodium azide,
50% Glycerol and 0.05% BSA. Stable for 12 months from date
of receipt