Flotillin-1 (FLOT1)_珈得尔化学试剂

【产品介绍】Flotillin-1, also known as Reggie-2, belongs
to a larger family of proteins that share an
evolutionarily conserved stomatin/prohibitin/
flotillin/HflK/C (SPFH) domain (1). Flotillin-1 is a
lipid raft-associated protein that has been implicated
in various cellular processes. Flotillin was first found
in cells that lack morphologically definable caveolae,
as well as in non-caveolar membranes and was
subsequently identified in low density detergent-
insoluble complexes of endothelial cells, where they
were termed flotillins (2, 3). As well as its
postulated role in neuronal regeneration, Flotillin-1
has been implicated in insulin signaling to trigger
glucose transporter redistribution in adipocytes
(4). Flotillin also associates with phagosomes
in macrophages (5).

Recommended Applications

WB, IHC, IP
Applications and Recommended Dilution Factors

WB: 1:10,000 - 50,000
IHC: 1:100 - 250
IP: 1:10 - 100
Species Reactivity *

Human, Mouse, Rat

*Species cross-reactivity is based on WB analysis

Products Data

Western blot analysis on (A) K562, (B) A431, (C) Jurkat,
and (D) HeLa cell lysates using anti-Flotillin-1
RabMAb (cat. #3868-1).

Immunohistochemical analysis of paraffin-embedded
human colon tissue using anti-Flotillin-1 RabMAb
(cat. #3868-1).

Immunohistochemical analysis of paraffin-embedded
human kidney tissue using anti-Flotillin-1 RabMAb
(cat. #3868-1).

Specificity

A synthetic peptide corresponding to residues on
the C-terminus in human Flotillin-1 was used as an immunogen.

贮存：

Storage Condition and Buffer

Store at -20° 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl,
40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for
12 months from date of receipt.