Recombinant Mouse Stem Cell Factor (P.pastoris-expressed) 是受体型蛋白酪氨酸激酶 KIT 的配体。在调节细胞存活和增殖，造血，干细胞维持，配子发生，肥大细胞发育，迁移和功能以及黑素生成中起重要作用。
Synonyms
rMuSCF; Hematopoietic growth factor KL; MGF; Mast Cell Growth Factor
Species
MouseSource
P. pastoris Accession
P20826 Gene ID
17311 Molecular Weight
Approximately 18.4 kDa AA Sequence
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA Biological Activity
The ED50 is <10.0 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of >1.0× 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Stem Cell Factor (P.pastoris-expressed) (rMuSCF) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Murine Stem Cell Factor (P.pastoris-expressed) (rMuSCF) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Embryo implantation is a complex process that requires the interaction of embryo and endometrium. Several growth factors and cytokines appear to be involved in this process. Stem cell factor (SCF) and its receptor c-kit regulate the proliferation and survival of germ cells and play an important role in follicular development. During embryonic development, SCF and c-kit are essential for the survival and proliferation of the germ cell and migration toward the gonad. c-kit mRNA is expressed in the primordial germ cells, while the SCF transcript is expressed along their migratory pathway toward the genital ridge[1]. |
