Recombinant Bovine Basic Fibroblast Growth Factory 有着广泛的生物学功能,包括有丝分裂发生、细胞存活、转移形成等。、 Synonyms rBobFGF; FGF-2; FGF2; FGF 2; HBGF-2 ; 重组牛碱性成纤维细胞生长因子 Species BovineSource E. coli Accession P03969 Gene ID 281161 Molecular Weight Approximately 16.5 kDa AA Sequence MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS Biological Activity The ED50 is <0.1 ng/mL as measured by murine Balb/c 3T3 cells, corresponding to a specific activity of >1.0 × 107 units/mg. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Bovine Basic Fibroblast Growth Factor (rBobFGF) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability Lyophilized recombinant Bovine Basic Fibroblast Growth Factor (rBobFGF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Fibroblast growth factor 2 (FGF2) is expressed in many cells and tissue types and has been reported to have a wide range of biological effects, including mitogenesis, cell survival, formation of metastases, remodelling and regeneration of tissues, control of cell proliferation and differentiation, regulation of a variety of genes and regulation of gene expression and rRNA transcription. Fibroblast growth factor 2 (FGF2) also plays an important role in fertility and early embryo development[1]. |