Recombinant Human Glial Cell Line-derived Neurotrophic Factor 是一种能有效促进多种神经元存活的小蛋白。
Synonyms
rHuGDNF; ATF ; 重组人胶质细胞源性神经营养因子
Species
HumanSource
E. coli Accession
P39905 Gene ID
2668 Molecular Weight
Approximately 17 kDa AA Sequence
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Biological Activity
The ED50 is <5 μg/mL as measured by C6 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Glial Cell Line-derived Neurotrophic Factor (rHuGDNF) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Human Glial Cell Line-derived Neurotrophic Factor (rHuGDNF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Glial Cell Line-derived Neurotrophic Factor (GDNF) is a small protein that potently promotes the survival of many types of neurons[1]. GDNF is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is a glycosylated disulfide-linked homodimeric protein of approximately 15 kDa. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells[1][2]. |
