Recombinant Rat Interleukin-18 具有免疫调节活性，包括协助Th1细胞和自然杀伤细胞刺激IFN-γ的生成，以及其它促进炎症的活动。
Synonyms
rRtIL-18; Interferon-gamma-inducing factor; IL-1 gamma ; 重组大鼠白细胞介素-18
Species
RatSource
E. coli Accession
P97636 Gene ID
29197 Molecular Weight
Approximately 18.4 kDa AA Sequence
MHFGRLHCTT AVIRSINDQV LFVDKRNPPV FEDMPDIDRT ANESQTRLII YMYKDSEVRG LAVTLSVKDG RMSTLSCKNK IISFEEMNPP ENIDDIKSDL IFFQKRVPGH NKMEFESSLY EGHFLACQKE DDAFKLVLKR KDENGDKSVM FTLTNLHQS Biological Activity
The ED50 is <2 μg/mL as measured by KG-1 cells, corresponding to a specific activity of >500 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris, 50 mM NaCl, pH 8.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Interleukin-18 (rRtIL-18) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Interleukin-18 (rRtIL-18) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin (IL)-18 is a potent stimulator of immunity and augments the severity of type II collagen-induced arthritis (CIA) in rats and mice by enhancing T helper 1 (Th1) cell activation, which increases the production of proinflammatory cytokines and arthritogenic antibodies. IL-18 possesses immunoregulatory activities, including the stimulation of interferon (IFN)-g production by T helper 1 (Th1) and natural killer cells, as well as other activities that promote inflammation[1]. |
