Recombinant Rat VEGF165 (CHO-expressed)是一个有效的血管生成调节剂，并能与信号蛋白受体neuropilin1 结合。
Synonyms
rRtVEGF165; VEGF-A; VPF ; 重组大鼠血管内皮生长因子165 (CHO 表达)
Species
RatSource
CHO Accession
QB5DEK7 Gene ID
83785 Molecular Weight
35-48 kDa AA Sequence
APTTEGEQKA HEVVKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCAGCC NDEALECVPT SESNVTMQIM RIKPHQSQHI GEMSFLQHSR CECRPKKDRT KPENHCEPCS ERRKHLFVQD PQTCKCSCKN TDSRCKARQL ELNERTCRCD KPRR Biological Activity
The ED50 is <4 ng/mL as measured by HUVEC cells, corresponding to a specific activity of >2.5 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat VEGF165 (CHO-expressed) (rRtVEGF165) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Rat VEGF165 (CHO-expressed) (rRtVEGF165) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Vascular endothelial growth factor (VEGF or VEGFA) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure[1]. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189[1][2]. VEGF165 binds the semaphorin receptor, Neuropilin1 and promotes complex formation with VEGF R2[3]. |
