Recombinant Human WISP-1 是一种能够促进肿瘤细胞血管生成的 WNT 诱导分泌蛋白。
Synonyms
rHuWISP-1; WNT1-inducible-signaling pathway protein 1; CCN4 ; 重组人源 WNT 诱导分泌蛋白
Species
HumanSource
CHO Accession
O95388 Gene ID
8840 Molecular Weight
44-46 kDa AA Sequence
TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LC Biological Activity
The ED50 is <0.5 μg/mL as measured by ATDC5 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human WISP-1 (CHO-expressed) (rHuWISP-1) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Human WISP-1 (CHO-expressed) (rHuWISP-1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
WNT-inducible secreted protein-1 (Wisp-1), a matricellular protein that promotes angiogenesis in cancers as well as cell survival in isolated cardiac myocytes and neurons, is a target of HDACs. Further, Wisp-1 transcription is regulated by HDACs and can be modified by the HDAC inhibitor, suberanilohydroxamic acid (SAHA/vorinostat), after MI injury[1]. |
