Recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed) 是在 HEK293 细胞中产生的 C-末端标记 IgG1 Fc 片段的多肽链。B7-2 (CD86) 是由抗原呈递细胞 (APC) 表达的共刺激分子，与 CD28 相互作用来促进 T 细胞活化和存活，并与 CTLA4 相互作用来进行免疫调节。
Synonyms
rHuB7-2/CD86, Fc Chimera; Activation B7-2 antigen; B70; BU63; CD86; CD28LG2
Species
HumanSource
HEK 293 Accession
P42081 Gene ID
942 Molecular Weight
65-80 kDa AA Sequence
LSGAAPLKIQ AYFNETADLP CQFANSQNQS LSELVVFWQD QENLVLNEVY LGKEKFDSVH SKYMGRTSFD SDSWTLRLHN LQIKDKGLYQ CIIHHKKPTG MIRIHQMNSE LSVLANFSQP EIVPISNITE NVYINLTCSS IHGYPEPKKM SVLLRTKNST IEYDGVMQKS QDNVTELYDV SISLSVSFPD VTSNMTIFCI LETDKTRLLS SPFSIELEDP QPPPDHIP Biological Activity
5 µg/mL (100 µL/well) of immoblized recombinant human B7-2/CD86-Fc can bind human Biotin-CD28-Fc with a linear range of 0.09-3.12 μg/mL. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed) (rHuB7-2/CD86, Fc Chimera) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human B7-2/CD86, Fc Chimera (HEK293-expressed) (rHuB7-2/CD86, Fc Chimera) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
CD86 is a transmembrane region, and a longer cytoplasmic domain than CD80. CD86 is constitutively expressed on interdigitating dendritic cells (DCs), Langerhans cells, peripheral blood DCs, memory B cells and germinal center B cells, and macrophages. CD86 is rapidly upregulated on B cells following activation by cross-linking of the Ig receptor or the addition of a variety of cytokines[1]. |
