Recombinant Human CD28, Fc Chimera (HEK293-expressed) 是在 HEK293 中产生的 C-末端标记 IgG1 Fc 片段的多肽链。CD28 是一种 T 细胞共刺激受体，是 PD-1 介导抑制的主要靶标
Synonyms
rHuCD28, Fc Chimera; TP44
Species
HumanSource
HEK 293 Accession
P10747 Gene ID
940 Molecular Weight
66-70 kDa AA Sequence
NKILVKQSPM LVAYDNAVNL SCKYSYNLFS REFRASLHKG LDSAVEVCVV YGNYSQQLQV YSKTGFNCDG KLGNESVTFY LQNLYVNQTD IYFCKIEVMY PPPYLDNEKS NGTIIHVKGK HLCPSPLFPG PSKP Biological Activity
2 µg/mL (100 µL/well) of immoblized recombinant human CD28-Fc can bind human Biotin-B7-1(CD80)-Fc with a linear range of 0.01-0.5 μg/mL. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human CD28, Fc Chimera (HEK293-expressed) (rHuCD28, Fc Chimera) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human CD28, Fc Chimera (HEK293-expressed) (rHuCD28, Fc Chimera) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
CD28 is the quintessential costimulatory molecule expressed on CD4+ and CD8+ T cells. During chronic infections and the normal aging process, CD28 expression is lost, compromising the functional activity of T cells. CD28 loss is promoted by replicative stress, particularly in the presence of tumor necrosis factor–α, owing to an inoperative CD28 initiator element. The co-receptor CD28 is strongly preferred over the T cell receptor (TCR) as a target for dephosphorylation by PD-1-recruited Shp2 phosphatase[1]. |
