Recombinant Human Heregulin-1 beta1 属于结构相关的多肽生长因子家族，来源于选择性剪接基因，诱导 Fn14 在 MCF7 细胞中表达。
Synonyms
rHuHeregulin-1 β1; ARIA; HRG; NRG1; Neuregulin-1 ; 重组人调蛋白-1 β1
Species
HumanSource
E. coli Accession
Q02297 Gene ID
3084 Molecular Weight
Approximately 7.6 kDa AA Sequence
MSHLVKCAEK EKTFCVNGGE CFMVKDLSNP SRYLCKCPNE FTGDRCQNYV MASFYKHLGI EFMEAE Biological Activity
The ED50 is <1 ng/mL as measured by MCF-7 cells, corresponding to a specific activity of >1 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Heregulin-1 beta1 (rHuHeregulin-1 β1) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Heregulin-1 beta1 (rHuHeregulin-1 β1) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
HRG1-β1 activates HER2/HER3 signaling and up-regulate Fn14 gene expression in parental MCF7 cells. HRG1-β1-mediated Fn14 up-regulation in MCF7/HER2-18 cells is required for maximal MMP-9 production[1]. |
