Recombinant Human Insulin-like growth factor-binding protein 4, His (HEK-expressed) 为胰岛素样生长因子结合蛋白家族一员，与胰岛素样生长因子 (IGF) 结合，介导 IGF 非依赖性生物活动。
Synonyms
rHuIGF-BP-4, His; IBP-4; IGFBP-4; BP4 ; 重组人胰岛素样生长因子结合蛋白 4 His (HEK 细胞表达)
Species
HumanSource
HEK 293 Accession
P22692 Gene ID
3487 Molecular Weight
30-35 kDa AA Sequence
DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFREHHH HHH Biological Activity
The ED50 is <50 ng/mL as measured by FDC-P1 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Insulin-like growth factor-binding protein 4, His (HEK-expressed) (rHuIGF-BP-4, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Insulin-like growth factor-binding protein 4, His (HEK-expressed) (rHuIGF-BP-4, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
The insulin-like growth factor-binding protein (IGFBP) family consists of six different protein members (IGFBP1-6) that show high inhibition for IGF-1 and IGF-2. IGFBP-4 is a smallest member and modulates the biologic functions by binding to IGFs. Moreover, IGFBP-4 mediates IGF-independent biologic activities. IGFBP-4 inhibits colorectal cancer, breast cancer and glioblastoma cells growth. IGFBP-4 acts a crucial inflammatory mediator in the occurrence of severities acute respiratory distress syndrome, which is responsible for cancer proliferation, migration and apoptosis[1]. |
