Recombinant Human Kallikrein-11 (Sf9 insect cells-expressed) 是一种可能的多功能蛋白酶，能有效地裂解激肽酶底物bz-Phe-Arg-4-methylcoumaryl-7-amide，并能较弱地裂解激肽酶和胰蛋白酶的其他底物。
Synonyms
rHuKallikrein-11; TLSP; hK11; Hippostasin; Trypsin-like protease; Serine protease 20 ; 重组人激肽释放酶11 (Sf9 昆虫细胞表达)
Species
HumanSource
Sf9 insect cells Accession
Q9UBX7 Gene ID
11012 Molecular Weight
Approximately 35.0 kDa AA Sequence
EFAATMLLVN QSHQGFNKEH TSKMVSAIVL YVLLAAAAHS AFAHHHHHHG SGSDDDDKET RIIKGFECKP HSQPWQAALF EKTRLLCGAT LIAPRWLLTA AHCLKPRYIV HLGQHNLQKE EGCEQTRTAT ESFPHPGFNN SLPNKDHRND IMLVKMASPV SITWAVRPLT LSSRCVTAGT SCLISGWGST SSPQLRLPHT LRCANITIIE HQKCENAYPG NITDTMVCAS VQEGGKDSCQ GDSGGPLVCN QSLQGIISWG QDPCAITRKP GVYTKVCKYV DWIQETMKNN Biological Activity
Specific activity determined by 100 µM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) is >2000 pmole/min/μg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Kallikrein-11 (Sf9 insect cells-expressed) (rHuKallikrein-11) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Kallikrein-11 (Sf9 insect cells-expressed) (rHuKallikrein-11) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed[1]. |
