Recombinant Human Nephroblastoma Overexpressed Gene Protein (CHO-expressed)，是一种血管生成剂，支持细胞粘附，诱导定向细胞迁移(趋化)，并促进细胞的存活。
Synonyms
rHuNephroblastoma Overexpressed Gene Protein; IBP-9; CCN3; IGFBP9; NOVH ; 重组人肾母细胞瘤过表达基因蛋白(CHO 表达)
Species
HumanSource
CHO Accession
P48745 Gene ID
4856 Molecular Weight
20-50 kDa AA Sequence
TQRCPPQCPG RCPATPPTCA PGVRAVLDGC SCCLVCARQR GESCSDLEPC DESSGLYCDR SADPSNQTGI CTAVEGDNCV FDGVIYRSGE KFQPSCKFQC TCRDGQIGCV PRCQLDVLLP EPNCPAPRKV EVPGECCEKW ICGPDEEDSL GGLTLAAYRP EATLGVEVSD SSVNCIEQTT EWTACSKSCG MGFSTRVTNR NRQCEMLKQT RLCMVRPCEQ EPEQPTDKKG KKCLRTKKSL KAIHLQFKNC TSLHTYKPRF CGVCSDGRCC TPHNTKTIQA EFQCSPGQIV KKPVMVIGTC TCHTNCPKNN EAFLQELELK TTRGKM Biological Activity
The ED50 is <5 μg/mL as measured by 3T3 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Nephroblastoma Overexpressed Gene Protein (CHO-expressed) (rHuNephroblastoma Overexpressed Gene Protein) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Nephroblastoma Overexpressed Gene Protein (CHO-expressed) (rHuNephroblastoma Overexpressed Gene Protein) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
CCN3 is expressed widely in derivatives of all three germ layers, and high levels of expression are observed in smooth muscle cells of the arterial vessel wall. Altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. CCN3 supports cell adhesion, induces directed cell migration (chemotaxis), and promotes cell survival[1]. |
