Recombinant Human OX40/TNFRSF4, His (HEK293-expressed) 通过与受体蛋白TRAF2和TRAF5的相互作用激活NF-kappaB。Recombinant Human OX40/TNFRSF4, His (HEK293-expressed) 促进凋亡抑制因子BCL2和BCL2lL1/BCL2-XL的表达，从而抑制细胞凋亡。
Synonyms
rHuOX40/TNFRSF4, His; ACT35 antigen; CD134; TXGP1L ; 重组人Human OX40/TNFRSF4, His (HEK293-表达)
Species
HumanSource
HEK 293 Accession
P43489 Gene ID
7293 Molecular Weight
40-45 kDa AA Sequence
LHCVGDTYPS NDRCCHECRP GNGMVSRCSR SQNTVCRPCG PGFYNDVVSS KPCKPCTWCN LRSGSERKQL CTATQDTVCR CRAGTQPLDS YKPGVDCAPC PPGHFSPGDN QACKPWTNCT LAGKHTLQPA SNSSDAICED RDPPATQPQE TQGPPARPIT VQPTEAWPRT SQGPSTRPVE VPGGRAVAHH HHHH Biological Activity
2 µg/mL (100 µL/well) of immoblized recombinant human OX40/TNFRSF4-His can bind human Biotin-OX40L-His with a linear range of 1.22-19.53 ng/mL. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human OX40/TNFRSF4, His (HEK293-expressed) (rHuOX40/TNFRSF4, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human OX40/TNFRSF4, His (HEK293-expressed) (rHuOX40/TNFRSF4, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
OX40/TNFRSF4 has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that OX40/TNFRSF4 promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis[1]. |
