Recombinant Human Sonic Hedgehog, C24IVI 是一种人重组 sonic hedgehog 的变体。Recombinant Human Sonic Hedgehog 是一种分泌信号转导器，不仅负责早期肢体和神经发育过程中前后轴的图形化，还负责在后期发育阶段产生细胞类型的多样性。
Synonyms
rHuSonic Hedgehog, C24IVI; HHG1 ; 重组人 Sonic Hedgehog, C24IVI
Species
HumanSource
E. coli Accession
Q15465 Gene ID
6469 Molecular Weight
Approximately 19.8 kDa AA Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Biological Activity
The ED50 is <2 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >500 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Sonic Hedgehog, C24IVI (rHuSonic Hedgehog, C24IVI) to 100 µg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Sonic Hedgehog, C24IVI (rHuSonic Hedgehog, C24IVI) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Sonic Hedgehog (SHH) is a secreted factor. The protein is heavily modified through cleavage and posttranslational modification and its release from producing cells can also be modulated by interactions with Dispatched, Scube2, and heparan sulfate proteoglycan[1][2]. |
