Recombinant Human Twisted Gastrulation, His 是带有标签的人重组 Twisted gastrulation。Recombinant Human Twisted Gastrulation 对预激活 T 细胞 (包括 IL-2、IL-4、IFN-γ 和 IL-10) 的 CD3 介导的增殖和细胞因子产生具有有效的抑制作用
Synonyms
rHuTwisted Gastrulation, His; TWSG1 ; 重组人扭曲原肠形成，His 标签
Species
HumanSource
HEK 293 Accession
Q9GZX9 Gene ID
57045 Molecular Weight
30-33 kDa AA Sequence
HHHHHHHHCN KALCASDVSK CLIQELCQCR PGEGNCSCCK ECMLCLGALW DECCDCVGMC NPRNYSDTPP TSKSTVEELH EPIPSLFRAL TEGDTQLNWN IVSFPVAEEL SHHENLVSFL ETVNQPHHQN VSVPSNNVHA PYSSDKEHMC TVVYFDDCMS IHQCKISCES MGASKYRWFH NACCECIGPE CIDYGSKTVK CMNCMF Biological Activity
The ED50 is <2 μg/mL as measured by ATDC-5 chondrogenic cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Twisted Gastrulation, His (rHuTwisted Gastrulation, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Twisted Gastrulation, His (rHuTwisted Gastrulation, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Twisted gastrulation (TSG) is one of the genes regulated by Tob and acts as an inhibitor of activated mature CD4+ human T lymphocytes. Recombinant Tsg had a potent inhibitory effect on CD3-mediated proliferation and cytokine production of preactivated T cells, including IL-2, IL-4, IFN-γ, and IL-10. Tsg is a potent agonist of TGF-β downstream signaling in activated human CD4+ T cells and suggest that enhancement of TGF-β mediating signaling by Tsg may represent a novel target for molecular intervention for control of aberrant T-cell responses during ongoing graft-versus-host disease (GVHD) and autoimmune diseases[1]. |
