Recombinant Human VEGF-121，一种血管内皮生长因子亚型剪接变体，不能与肝素结合。Recombinant Human VEGF-121 是活性 B 细胞样弥漫性大 B 细胞淋巴瘤生存的预测因子，与保存在癌症中的免疫应答基因特征有关。
Synonyms
rHuVEGF-121; VEGF-A; VPF ; 重组人血管内皮生长因子-121
Species
HumanSource
E. coli Accession
P15692 Gene ID
7422 Molecular Weight
Approximately 28.2 kDa AA Sequence
MPMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKCDKPR R Biological Activity
The ED50 is <5 ng/mL as measured by HUVEC cells, corresponding to a specific activity of >2 × 105/sup> units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human VEGF-121 (rHuVEGF-121) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human VEGF-121 (rHuVEGF-121) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
VEGF-121 lacks heparin binding ability and requires cell-surface heparan sulfates for efficient binding to the VEGF receptors of human melanoma cells[1]. In addition, VEGF-121 and VEGF 165 regulate blood vessel diameter through vascular endothelial growth factor receptor 2 in an in vitro angiogenesis model. VEGF-121 is predictor for survival in activated B-cell-like diffuse large B-cell lymphoma and is related to an immune response gene signature conserved in cancers[2][3]. Furthermore, VEGF-121 plasma level is biomarker for response to anti-angiogenetic therapy in recurrent glioblastoma[4]. |
