Recombinant Human Ameloblastin, His (HEK293-expressed)，一种在 HEK293 细胞中产生的重组人成釉细胞素，在 C 末端有 His 标记。Recombinant Human Ameloblastin 是一种釉质基质蛋白，通过分化成釉细胞表达。
Synonyms
rHuAmeloblastin, His; Ameloblastin; AMBN ; 重组人成釉细胞素，His (HEK293-expressed)
Species
HumanSource
HEK 293 Accession
Q9NP70 Gene ID
258 Molecular Weight
Approximately 66.0 kDa AA Sequence
LQSAAATTNQ ATALKEALQP PIHLGHLPLQ EGELPLVQQQ VAPSDKPPKP ELPGVDFADP QGPSLPGMDF PDPQGPSLPG LDFADPQGST IFQIARLISH GPMPQNKQSP LYPGMLYVPF GANQLNAPAR LGIMSSEEVA GGREDPMAYG AMFPGFGGMR PGFEGMPHNP AMGGDFTLEF DSPVAATKGP ENEEGGAQGS PMPEANPDNL ENPAFLTELE PAPHAGLLAL PKDDIPGLPR SPSGKMKGLP SVTPAAADPL MTPELADVYR TYDADMTTSV DFQEEATMDT TMAPNSLQTS MPGNKAQEPE MMHDAWHFQE PHHHHHH Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Ameloblastin, His (HEK293-expressed) (rHuAmeloblastin, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Ameloblastin, His (HEK293-expressed) (rHuAmeloblastin, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Ameloblastin, also known as amelin or sheathlin, is the most abundant non-amelogenin enamel matrix protein. As shown in AMBN-null mice, AMBN is important for the attachment, polarity, proliferation, and differentiation of ameloblasts. Ameloblastin is a cell adhesion molecule essential for amelogenesis, and it plays a role in maintaining the differentiation state of secretory stage ameloblasts by binding to ameloblasts and inhibiting proliferation[1][2]. |
