Recombinant Human Apolipoprotein A-II, His (HEK293-expressed) 在 HEK293 细胞中表达，N 端带有一个 His 标签。Apolipoprotein A-II (ApoA-II) 与 HDL 代谢的许多方面有关，并且 apoA-II 过表达产生促动脉粥样硬化表型。
Synonyms
rHuApolipoprotein A-II, His; ApoA2; Apolipoprotein A-II
Species
HumanSource
HEK 293 Accession
P02652 Gene ID
336 Molecular Weight
10-15 kDa AA Sequence
QAKEPCVESL VSQYFQTVTD YGKDLMEKVK SPELQAEAKS YFEKSKEQLT PLIKKAGTEL VNFLSYFVEL GTQPATQHHH HHH Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Apolipoprotein A-II, His (HEK293-expressed) (rHuApolipoprotein A-II, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Apolipoprotein A-II, His (HEK293-expressed) (rHuApolipoprotein A-II, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant apoA-II is comparable to the plasma form in its ability to bind and reorganize lipid and promote cholesterol efflux from macrophages via the ATP binding cassette transporter A1. Synthesized primarily in the liver, apoA-II is a 77 amino acid protein with a single cysteine at position. In healthy humans, apoA-II exists almost entirely as a disulfide-linked homodimer (molecular mass, 17.4 kDa), though it can be occasionally found as a heterodimer with other Cys-containing proteins[1]. |
