Recombinant Human Apolipoprotein A-IV, His (HEK293-expressed) 在 HEK293 细胞中表达,N 端带有一个 His 标签。Apolipoprotein A-IV (Apo A-IV) 在胆固醇逆向转运中起作用。 Synonyms rHuApolipoprotein A-IV, His; ApoA4; Apolipoprotein A-IV Species HumanSource HEK 293 Accession P06727 Gene ID 337 Molecular Weight Approximately 46.0 kDa AA Sequence EVSADQVATV MWDYFSQLSN NAKEAVEHLQ KSELTQQLNA LFQDKLGEVN TYAGDLQKKL VPFATELHER LAKDSEKLKE EIGKELEELR ARLLPHANEV SQKIGDNLRE LQQRLEPYAD QLRTQVNTQA EQLRRQLTPY AQRMERVLRE NADSLQASLR PHADELKAKI DQNVEELKGR LTPYADEFKV KIDQTVEELR RSLAPYAQDT QEKLNHQLEG LTFQMKKNAE ELKARISASA EELRQRLAPL AEDVRGNLRG NTEGLQKSLA ELGGHLDQQV EEFRRRVEPY GENFNKALVQ QMEQLRQKLG PHAGDVEGHL SFLEKDLRDK VNSFFSTFKE KESQDKTLSL PELEQQQEQQ QEQQQEQVQM LAPLESHHHH HH Biological Activity Data is not available. Appearance Lyophilized powder. Formulation Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2. Endotoxin Level <1 EU/μg, determined by LAL method. Reconstitution Reconstitute the lyophilized recombinant Human Apolipoprotein A-IV, His (HEK293-expressed) (rHuApolipoprotein A-IV, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability Lyophilized recombinant Human Apolipoprotein A-IV, His (HEK293-expressed) (rHuApolipoprotein A-IV, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping Room temperature in continental US; may vary elsewhere. Background Human apolipoprotein (apo) A-IV, a 46 kd protein, is capable of serving as a cofactor for the enzyme lecithin: cholesterol acyltransferase (LCAT), to bind to apo A-I/A-II receptor sites on mouse adipose cells and thereby promote cellular cholesterol efflux[1]. |