Recombinant Human Apolipoprotein H, His (HEK293-expressed) 在 HEK293 细胞中表达，N 端带有一个 His 标签。Apolipoprotein H (ApoH) 是在许多系统性红斑狼疮和抗磷脂综合征 (APS) 患者中发现的抗磷脂抗体 (aPLs) 的主要靶标。
Synonyms
rHuApolipoprotein H, His; ApoH; B2G1; B2GP1; Apolipoprotein H
Species
HumanSource
HEK 293 Accession
P02749 Gene ID
350 Molecular Weight
45-70 kDa AA Sequence
GRTCPKPDDL PFSTVVPLKT FYEPGEEITY SCKPGYVSRG GMRKFICPLT GLWPINTLKC TPRVCPFAGI LENGAVRYTT FEYPNTISFS CNTGFYLNGA DSAKCTEEGK WSPELPVCAP IICPPPSIPT FATLRVYKPS AGNNSLYRDT AVFECLPQHA MFGNDTITCT THGNWTKLPE CREVKCPFPS RPDNGFVNYP AKPTLYYKDK ATFGCHDGYS LDGPEEIECT KLGNWSAMPS CKASCKVPVK KATVVYQGER VKIQEKFKNG MLHGDKVSFF CKNKEKKCSY TEDAQCIDGT IEVPKCFKEH SSLAFWKTDA SDVKPCHHHH HH Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.2. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Apolipoprotein H, His (HEK293-expressed) (rHuApolipoprotein H, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Apolipoprotein H, His (HEK293-expressed) (rHuApolipoprotein H, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Apolipoprotein H (ApoH), also known as β2-glycoprotein I (β2GPI), is a 43–50 kDa single-chain glycoprotein that is expressed predominantly in the liver but also in other cell types such as endothelial cells, lymphocytes, astrocytes and neurons. ApoH is a phospholipid-binding (e.g. cardiolipin) plasma protein that also binds other negatively charged molecules such as DNA and oxidised low-density lipoproteins and interacts with a variety of proteins[1]. |
