Recombinant Human Apolipoprotein A-II, His (HEK293-expressed) 在 HEK293 细胞中表达，N 端带有一个 His 标签。稳定表达 apoE3 或 apoE4 (HEK-apoE) 的 HEK 细胞分泌的 Apolipoprotein E (apoE) 结合 Aβ，并抑制 Aβ 诱导的神经毒性。
Synonyms
rHuApolipoprotein M, His; ApoM; Apolipoprotein M
Species
HumanSource
HEK 293 Accession
O95445 Gene ID
55937 Molecular Weight
Approximately 26.0 kDa AA Sequence
MFHQIWAALL YFYGIILNSI YQCPEHSQLT TLGVDGKEFP EVHLGQWYFI AGAAPTKEEL ATFDPVDNIV FNMAAGSAPM QLHLRATIRM KDGLCVPRKW IYHLTEGSTD LRTEGRPDMK TELFSSSCPG GIMLNETGQG YQRFLLYNRS PHPPEKCVEE FKSLTSCLDS KAFLLTPRNQ EACELSNNHH HHHH Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Apolipoprotein M, His (HEK293-expressed) (rHuApolipoprotein M, His) to 100 µg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Apolipoprotein M, His (HEK293-expressed) (rHuApolipoprotein M, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Apolipoprotein E (apoE) is a 34-kDa glycoprotein that is a surface component of various plasma lipoprotein particles including chlyomicron remnants, β-migrating VLDL (β-VLDL), LDL, and a subclass of HDL. ApoE mediates high-affinity binding of apoE-containing lipoproteins to cell surface endocytic receptors during the transport and metabolism of plasma cholesterol (Chol) and triglycerides (TG). HEK-apoE is a ligand for low-density lipoprotein (LDL) receptor-related protein (LRP) but not the LDL receptor[1]. |
